The aim of this research was to develop a process for making an inoculum from cow dung. Consequently the inoculum together with food waste was employed to produce hydrogen gas. A total of four different inoculums were experimented including 1). Fresh inoculum 2). Autoclaved inoculum 3). Natural dried inoculum and 4). Hot air dried inoculum. The experimental results revealed that the autoclaved inoculum is the most effective in terms of producing a volatile fatty acid in a maximum value of 9.55 g/l. In addition a maximum hydrogen gas of 136.46 ml/g (of sugar) and also having an average pH of 4.85 and the sugar concentration of 0.33 g/l could be obtained. Furthermore it was found that creating an inoculum by using the autoclaved inoculum produces an inoculum powder having a concentration of 20%. This suggests that the autoclaved inoculum is the best in terms of producing the hydrogen gas from using food waste is 35 litre/litre (of food waste).