Honey-nanofibers were fabricated by electrospinning technique in order to study their potentiality for biomedical application especially as wound healing materials. Honey/Gelatin ratios operating voltages and solution flow rates were considered as the experimental variables. All synthesized nanofibers were tested for their sizes percentages of water uptake and contact angles between the fiber surfaces and water droplets. It was found that 1) size of nanofibers and contact angle between the fiber surfaces and water droplet increased with increasing of honey in solution 2) size and percentage of water uptake of nanofibers increased with stepping up of operating voltage and 3) size of nanofibers was minimized at the flow rate of 0.06 ml/hr for each operating voltage of this study.