The objective of this research were to focus the antioxidant activity and separated the chemical compound of crude extract from Feronia limonia Swingle branch. After that the optimum antioxidant of crude extract was used to prepare the antioxidant lotion. The result showed that crude extract had total phenolic and total tannin contents 41.99 and 52.35 g/ml ; respectively and also had antioxidant activity (IC50) 12.55 ?g/ml Whereas BHT and BHA had IC50 8.96 and 9.61 ?g/ml. The separation of crude extract can be separated into four groups (F1 F2 F3 F4) and the results showed that F2 has the strongest activity against the others with IC50 = 9.53 ?g/ml. Then F2 was analyzed with GC-MS and found that the structure were Hexadecanoic acid Ethyl ester Trimethylsilyl ester and Dioctyl ester. The lotion was odorless white color with no phase separation with pH 8.0