In this study The paper aims to produce extracts from guava leaves. For use in food packaging. And to test their ability to inhibit bacteria. Staphylococcus aureus paper extracts from guava leaves. Because the bacteria Staphylococcus aureus to live in the air dust waste water food and milk supplies. The strain also grows well in climates with oxygen. The test extracts from guava leaves. A chemical properties of guava leaf extract has a pH of 5.4 + 0.02 acid content (% citric acid) was 1.2 ± 0.1 and the amount of phenolic total (mg gallic acid / g) levels. 63.1: 1.9 The performance of guava leaf extract in inhibiting bacteria. Staphylococcus aureus by means paper disc diffusion on agar Nutrient agar at 36 ° C for 24 hours diameter clear zone (cm.) Was 1.3 ± 0.5 and tested for the Minimum Inhibitory Concentration (MIC) by Broth Dilution Technique with. equal to 1.56% of extract of guava leaves