This independent study was aimed to 1) explore the users’ demographic characteristics 2) level of perception of service quality and 3) comparison of demographic characteristics differences of the perceived service quality at Hua Chiew Thai – Chinese traditional Thai medication. The samples used in the research were 400 service users of Hua Chiew Thai – Chinese traditional Thai medication. The questionnaire was applied for the data collection. The statistics used for data analysis were descriptive statistics including frequency percentage mean standard deviation t-test F-test and the analysis of One-Way ANOVA. When the statistical significance. 0.05 was found multiple comparison test using Scheffes method was applied. The study results revealed that the users who differed in terms of demographic characteristics education level and marital status perceived the overall service quality differently.