This research aims 1) to study the relationship of human capital development to promote Thai traditional medicine in central north-eastern region 2) to analyze factors effect to human capital development to promote Thai traditional medicine in central north-eastern region 3) to synthesize the relationship of human capital development to promote Thai traditional medicine in central north-eastern region 4) to synthesize the quality of human capital to promote Thai traditional medicine in central north-eastern region. Research methodology is mixed-method in both quantitative and qualitative. Quantitative analysis data collected were 370 samplings from Thai traditional medicine in central north-eastern region; 4 provinces Kalasin Roi Et Mahasara Kham and KhonKaen between August 2017 – April 2018. Statistical analysis was by canonical method for testing dependent and independent variables. Sample was calculated under Krejcie and Morgan (Krejcie and Morgan 1970:608) against 1543 Thai traditional medicines in central north-eastern region. IOC for questionnaire testing validity and reliability were with alpha coefficient more than 0.70. Data analyzed by SPSS 20 calculated mean median mode standard deviation t-test and F-test statistics analysis using canonical correlation test for dependent and independent variables relation. Qualitative analysis data collected by in-depth interview from administrative of Thai Herbal institution the administrative of center of Thai traditional medicine for gathering and examining the quantitative previous data. The results are 1) human capital development has related to Thai traditional medicine for medical treatment in Thai traditional medicine course of study in low range 2) human capital development has related to Thai traditional medicine for medical treatment for practice experience in very low range 3) human capital development has related to Thai traditional medicine for medical treatment in Thai traditional medicine professional status in low range 4) factors influence to human capital development to promote Thai traditional medicine in medical treatment are government supporting policies and remuneration system 5) human capital development has related to Thai traditional medicine for medical treatment in knowing sickness knowing medicine capability of medical knowledge and knowing sickness originated that has related to course of study and certificate granted in medical treatment pharmacy midwifery and Thai massage in high range 6) human capital development has related to Thai traditional medical evolution in government supporting policies and remuneration in very high range.