This research has studied the treatment of municipal wastewater with shoe soles as a supporting media for Sequencing Batch Reactor. The objectives of this research were the conditions to for optimize growing Photosynthetic Bacteria (PSB) and understand the treatment efficiencies of municipal wastewater treatment with PSB in Sequencing Batch Reactor (SBR). Three types of shoe soles were applied in this research composed of rubber shoe soles neoprene and EVA and the control without shoe soles. The experimental factors included the essential parameters such as BOD COD SS MLSS MLVSS Phosphorus and Ammonia-Nitrogen. F/M Ratio was adjusted at 0.13 and hydraulic retention time (HRT) was at 5 days. The results indicated that the removal efficiencies of SBR with rubber shoe soles in terms of BOD COD SS phosphorus and ammonia-nitrogen were 87% 80% 94% 53% and 68% respectively. The removal efficiencies of SBR with Neoprene shoe soles in terms of BOD COD SS Phosphorus and Ammonia-Nitrogen were 89% 81% 95% 20% and 73%. The removal efficiencies of SBR with EVA shoe soles in terms of BOD COD SS phosphorus and ammonia-nitrogen were 91% 86% 95% 56% and 74%. The removal efficiencies of SBR without shoe soles in terms of BOD COD SS phosphorus and ammonia-nitrogen were 84% 79% 93% 86% and 67% respectively. The treated wastewater was rather clear low content of suspended solids and no malodor. From overall results the treatment of municipal wastewater by anaerobic sequencing batch reactor with different types of shoe soles was not significant for wastewater treatment efficiencies by anaerobic with any 3 types among of shoes soles can be efficiently applied for municipal wastewater treatment.