The biological activity of erude hexane ethylacetate and methanol extracts from stem bark of Xylocarpus granatum Koen. Walsura trichostemon Miq. And Parinari anamense Hance with sequential extraction and maceration technique. The crude extract was employed toevaluate the biological activity such as the antimicrobial activities test by the Microplate broth dilution techniques the antioxidant activities test by DPPH radical scavenging assay the totalphenolic contents test by the Folin-Ciocalteu method the anti-malaria (Plasmodium falciparum) test by Microculture Radioisotope Technique the anti-Mycobacterium Tuberculosis (anti-TB) test by Green fluorescent protein microplate assay (GFPMA) and the anti-cancer (KB-Oral cavity cancer) test by Resazurin Microplate assay (REMA)