The objective of this study is to obtain a model that maximize production of γ-linolenic acid (GLA) by the moss Physcomitrella patens employing response surface methodology (RSM). The RSM with a three-variables (sucrose potassium nitrate and glutamate concentrations) and five-level (-2 -1 0 1 and 2) central composite design (CCD) including 20 experimental runs was employed to optimize the medium composition. Results showed that the experimental data could be appropriately fitted into a second-order polynomial model with a coefficient of determination (R2) more than 0 .9 5 for GLA production. Analysis of variance (ANOVA) revealed that the model was highly significant (p<0.0001) and the effects of the sucrose (20-100 g/L) and glutamate (0.5-2.5 g/L) concentrations on GLA production were significant (p<0.05). The GLA production with the optimized culture medium (sucrose concentration of 62.92 g/L potassium nitrate of 0.80 g/L and glutamate concentration of 1 .4 2 g/L) by RSM increased 4.61 folds when compared with the standard BCD medium. This experimental value (16.37 mg GLA/L) fit well with the predicted values (16.20 mg GLA/L).

. In this study examination of label information physical and microbiological qualities was performed in 30 clear-plastic and 3 opaque-plastic bottled drinking water products distributed in city municipality of Ubon Ratchathani. Results showed that 12 clear-plastic (36%) and 3 opaque-plastic (9%) bottled drinking water samples had no expiry date on the product labels which were discordant to the criteria for drinking water in sealed containers imposed by the Ministry of Public Health of Thailand no. 383 (2017). Odorless appearances of all drinking water samples were observed. Values of pH in 4 clear-plastic (12%) and 3 opaque-plastic (9%) bottled drinking water samples were in the ranges of 6.21±0.00–6.29±0.03 which were below the allowable limit (pH 6.5–8.5). All bottled drinking water products contained numbers of total coliform and fecal coliform bacteria less than 2 MPN/100 mL and were devoid of E. coli. Therefore based on the quality of clear-plastic and opaqueplastic bottled drinking water products distributed in city municipality of Ubon Ratchathani 1 5 samples (45%) did not meet standard for drinking water in sealed containers declared by the Ministry of Public Health of Thailand (1981 and 2017) owing to no expiry date labeled on the products and pH value below 6.5.

. This research aimed to study the acidification processes of carving vegetable using acid conditioning machine under emperature and pressure control which was developed in this study. Four acidification procedures were done at normal atmospheric pressure (method 1) under vacuum pressure for 10 (method 2) and 20 min (method 3) and vacuum pressure alternating with normal atmospheric pressure for each of 1 hour. There were two main parts of acid conditioning machine including the temperature controller inside the pickle tank and heat conductor tank and the pressure controller inside the pickle tank. The pickling temperature was stabled at 40oC under the pressure of 0.5 Bar. PLC (Programmable Logic Controller) program was used to write the ladder for controlling all processes and display through SCADA system. The results showed that the acidification method by soaking the carved-rose carrot under vacuum pressure alternating with normal atmospheric pressure showed the shortest time to equilibrium by 7 hours. Then the carved-rose carrot was packed in the glass jar followed by sterilized in boiling water for 15 minutes and stored at room temperature for 7 days. Total bacteria count Escherichia coli and Yeast-mold were not exceeded than the standard limit. Staphylococcus aureus was not detected throughout the storage periods

The objective of this research was to find the suitable processes of carved-vegetables which packed in hermetically sealed container. Two vegetables were composed of carrot and pumpkin. There were carved into rose or leaf shape. An optimum ratio of 2 recipes of pickle solution were studied. The first one was a recipe which acidified with lactic acid (acidified-recipe) to pH <4.6. Another one was a recipe supplemented with preservative (preservative-added recipe). The results showed that an optimum ratio for acidified-recipe was composed of 7.23% vinegar 64.81% water 0.81% salt 27.00% sugar and 0.15% lactic acid which showed the final pH was 3.27. For preservative-added recipe an optimum ratio was composed of 27.03% vinegar 27.03% water 0.9% salt 45.05% sugar and 200 ppm sodium metabisulfite which showed the final pH was 3.39. Carrot pumpkin green papaya and cucumber were sanitized in peroxyacetic acid (80 mg/L 3 min). Radish was dipped in tap water for 3 min. Then all carved-vegetables were immersed in calcium chloride solution (0.5% 2 min) before preserved in acidified-recipe solution in plastic box with a lid and then stored at room temperature for 4 days. Equilibrium time pH and °Brix were studied. The results showed that pH and °Brix were decrease during 4 days storage and showed obviously tended to equilibrium. However carved-radish and cucumber were spoiled by microorganisms as a film on the surface of pickle solution and gas bubble was occurred inside a container on 4 days of the storage. Hence three carved-vegetables including carrot pumpkin and radish were used in the next experiment which preserved in preservative-added recipe. Treatment was compared with the same recipe without preservative as the control. All types of carved-vegetables had an equilibrium time for 7 days. Three parts of samples e.g. pickle solution carved-vegetables and mixed-sample showed pH lower than 4.6 and °Brix were gradually decreased during the storage. Shelf-life of all carved-vegetables stored in hermetically sealed container was up to 7 days. Total bacteria count E. coli yeast-mold were not exceeded than the standard limited by Ministry of Public Health and Staphylococcus aureus was not detected throughout 7 days of the storage.

Nowadays the measurement technologies are continually growth in advanced. These can be seen in the developed and developing countries that research and develop the products which support the consumer behavior and/or country economy ic growth. However some advanced products are very expensive such as pH meter. Therefore this thesis develops the pH meter which allows the acid-base test of the water and it can be supported the agriculture. The proposed pH meter is included the pH pen sensor temperature sensor water level sensor. The proposed meter can measure the acid-base of the water by sending SMS alert signal via GSM module to the mobile phone of the farmer. As can be seen this thesis can prevent and solve the effect of the high acid-base of the water that possibly flows into the agriculture farm via using SMS alert signal of the mobile phone of the farmer.