Nitrogen is an essential nutrient for plant growth. When the plants have been grown for a long-term effect to decreased nitrogen in soil and insufficiency of the plant growth. One biological method for increasing the nitrogen content in soil is utilize of free nitrogen-fixing bacteria Azotobacter. Therefore the objective of this research was to search natural material suitable for Azotobacter fixation. The 10 natural materials were observed physical structure and investigated the ability of natural materials for Azotobacter fixation. The results showed that water hyacinth and bagasse stalks were fixed the Azotobacter of 2.43x108 and 1.94 x108 CFU per gram of each natural material respectively. After that the selected fixed Azotobacter of water hyacinth and bagasse stalks were studied the growth of Brassica Chinensis. This experiment was designed to 4 treatments. The first treatment was mixed of water hyacinth stalks and the Azotobacter. Second treatment was mixed of bagasse stalks and the Azotobacter. The third treatment was the Azotobacter only. The last treatment was soil only. The results indicated that the average of plant growth include height leaf number root length fresh weight and dry weight of the mixed of water hyacinth stalks and the Azotobacter had significantly higher growth rates than other treatments. The total nitrogen was also increased 1.4 fold in the soil. All results suggest that the water hyacinth stalk is alternative for the fixation of the free nitrogen-fixing bacteria Azotobacter as induce nitrogen in the soil and plant growth promotion.

Premna trichostoma Miq. (Lamiaceae) mainly distribute in Asia including Thailand. Folk healers use this plant to treat fever as a juice splash and bathe. The aims of this study are to evaluate the quantity of active constituents and antioxidant activity of P. trichostoma leaf extract. Total phenolic compounds were determined using Folin-Ciocalteu method. Total flavonoid contents were determined by Aluminum chloride method. The amount of apigenin and kaempferol contents were analyzed by high performance liquid chromatography (HPLC). In addition the antioxidant activity was evaluated by DPPH and ABTS assay. The results showed that total phenolic contents total flavonoid contents and the amount of apigenin and kaempferol of P. trichostoma leaf extract was 46.45±15.30 mg GAE/g extract 25.48±2.02 mg CE/g extract 11.16±0.04 and 4.54±0.08 µg/g extract respectively. The IC50 values from the DPPH and ABTS assay was 117.50±1.22 and 36.14±1.53 µg/ml respectively. This study shows the antioxidant properties and active constituents of P. trichostoma leaf extract which indicates the potentials for further development of this plant

The objective of this study is to obtain a model that maximize production of γ-linolenic acid (GLA) by the moss Physcomitrella patens employing response surface methodology (RSM). The RSM with a three-variables (sucrose potassium nitrate and glutamate concentrations) and five-level (-2 -1 0 1 and 2) central composite design (CCD) including 20 experimental runs was employed to optimize the medium composition. Results showed that the experimental data could be appropriately fitted into a second-order polynomial model with a coefficient of determination (R2) more than 0 .9 5 for GLA production. Analysis of variance (ANOVA) revealed that the model was highly significant (p<0.0001) and the effects of the sucrose (20-100 g/L) and glutamate (0.5-2.5 g/L) concentrations on GLA production were significant (p<0.05). The GLA production with the optimized culture medium (sucrose concentration of 62.92 g/L potassium nitrate of 0.80 g/L and glutamate concentration of 1 .4 2 g/L) by RSM increased 4.61 folds when compared with the standard BCD medium. This experimental value (16.37 mg GLA/L) fit well with the predicted values (16.20 mg GLA/L).

This research was resulted of the characterization of cement paste composites (CPC) blended with palmyra fiber ash and surface-treated on short SWNT prepared by an electroless plating process. The cement paste composites included cement 270 meshes of palmyra fiber ash and short SWNT. The design of experiment was divided into 3 conditions namely uncuring 28 and 56 days in distilled water. The properties of CPC were carried out in chemical composition physical mechanical mineral phase microstructure and statistical analysis. It was found that in the chemical compositions an amount of silicon dioxide aluminium oxide and ferric oxide were 51.53% which was rated in class C. The bulk densities of CPC series between PFA10 (C) and PFA5AgSWNT0.01 (G) cured 56-day distilled water were an increased 38%. Then the highest specific electrical resistivity of CPC series PFA5OSWNT0.03 (F) cured 56-day distilled water was 1.14±0.31 mega-ohm.mm. The highest compressive strength of CPC series PFA5AgSWNT0.01 (G) cured at 28-day distilled water was 25.45±2.00 MPa compared with the OPC (A) was an increased 5%. Which the bulk densities of CPC series between PFA10 (C) and PFA5AgSWNT0.01 (G) were statistical analyzed the mostly cured at significant difference α = 0.05. In addition CPC series PFA5AgSWNT0.01 (G) mainly contained calcite portlandite quartz dolomite and ettringite were caused increasing compressive strength. And the SEM microphotograph were also displayed the calcium silicate hydrate portlandite and ettringite. Consequently the PFA was used to ability pozzolanic material using for lightweight concrete of CPC.

Young coconut shells are a byproduct after processing coconut juice which can be used to produce a solid-fuel. This fuel from the coconut shell is an alternative source instead of using firewood or charcoal made of timber in the natural forest. The solid-fuel from young coconut shells was prepared by using a wet glue powder as a binder then was compressed by using a briquette machine. The black coconut briquette was stable after exposure to the sun. The average of the briquette combustion rate was at 11.24 g /min. There is no eruption smut smoke and odor by using the young coconut shell briquette yo start a fire. To sum up a young coconut shell has a high potential as an alternative biomass material for producing a briquette especially for commercial. This solution can increase the reduction of haze weather in the sight of solid waste incineration and also global warming.