The potential health benefits attributed to mulberry leaf tea and its bioactive compounds such as antioxidant effects cancer chemoprevention and lower blood sugar levels have led to a huge increase of mulberry leaf tea products in the food market. The objectives of this research were to determine the appropriate amount of mulberry leaf tea powder Buri Rum 60 added in 4 snack products which were cook by 4 different process i.e. bread (baked) puifai (steam) peakpoon (stir) and snow lotus (no heat treatment). The Mulberry leaf tea powder was used in the ingredients for 3 levels i.e. 5 10 and 15%. Then the most preferred formula was analyzed for the nutritional values. The result showed that all 4 snacks added with Mulberry leaf tea powder at level of 10% were accepted the most. The nutritive value of these 4 snacks indicated that the moisture contents decreased while protein fiber ash energy increased significantly (p≤0.05). In comparison to the original formulas fat decreased significantly in bread and snow lotus added with mulberry leaf tea powder (p≤0.05) and decreased non-significantly in puifai and peakpoon added with Mulberry leaf tea powder (p>0.05). Carbohydrate increased significantly in bread and peakpoon added with mulberry leaf tea powder (p≤0.05) and increased non-significantly in puifai and snow lotus added with Mulberry leaf tea powder (p>0.05).

The objectives of this research were to characterize growth and melanin production on different culture media effect of copper ion and cobalt ion on growth and melanin production including antibacterial activity of crude melanin. The results revealed that actinomycetes strain PNST-01 have good growth on ISP-7 medium synthetic medium while strain PRBS-12 grow very well on ISP-3 medium complex medium. Both strains produced high amount of melanin showing brownish colony on ISP-7 medium but there is no melanin pigment production on ISP-1 medium and ISP-6 medium. Moreover the growth rate was reduced and both strains could not produce melanin pigment on ISP-7 medium without supplement with copper chloride and cobalt chloride. Crude melanin extract of strain PNST-01 showed antimicrobial activity against Bacillus cereus ATCC 11778 Bacillus subtilis ATCC 6633 and Staphylococcus aureus ATCC 25923 but could not inhibit Escherichia coli Shigella dysenteriae Salmonella typhi Klebsiella pneumoniae Serratia marcescens Pseudomonas aeruginosa and Streptococcus pyogenes . While crude melanin extract of strain PRBS-12 gave antimicrobial activity only on B. cereus ATCC 11778 and B. subtilis ATCC 6633. Based on the study the results can be used as key information for further development of melanin production and application in various industries.

Border crossing trade can an important role in the competitiveness of Thai products in neighboring countries as Thailand has a geographical advantage that connects with neighboring countries making border trade an important part of generating revenue for the country. Thailand - Malaysia border trade study showed that the transit process took a long time resulting in a waiting truck at the checkpoint causing traffic jams and the congestion and congestion of the customs. As a result the average waiting time of the system takes up to 264.19 minutes per trip and transportation cost from 7141.87 baht per trip by reducing the waiting time from the process to 13.41 minutes per trip it was found that the transportation cost was 6975.16 baht per trip (Decrease of 166.71 baht). Reduction the transportation costs and waiting times enhance the potentiality and the competitiveness of border crossing

This research aims to increasing efficiency in production and eliminate the problem of insufficient production capacity with respect to customer demand for electrical appliance factory by applying the line balancing method. Thus the study then developed two problem-solving approaches. The first approach is a method by which a firm applies the line balancing concept and ECRS technique (Eliminate Combine Rearrange and Simplify). On the contrary the second approach is required an investment in testing machine with value of 1000000 baht in order to support sufficient production capacity to meet customer demand. Also ECRS method is used to accomplish this process. Based on the results the first approach provided the production line efficiency improvement representing of 82.59% and reduced the labor cost of 277220 baht per year. However the loss of commercial opportunity has remained the same of 6336000 baht per year. Accordingly the second approach demonstrated of 92.11% of the production line efficiency improvement and the labor cost reduction valued of 277220 baht per year. As we expected the second approach satisfied production efficiency by eliminating the loss of commercial opportunity with value of 6336000 baht per year delivering a return of investment within two months.

The objective of this research was to isolate plant growth promoting rhizobacteria from Wedelia trilobata (L.) A.S. Hitchcock rhizosphere and preliminarily test their plant growth promoting properties such as nitrogen fixation siderophore production phosphate solubilization and indole-3-acetic acid production. Forty-seven bacterial isolates were obtained including 26 isolates of gram positive 18 isolates of gram negative and 3 isolates of actinobacteria. After that 8 isolates (including BWTM1_MSU BWTM2_MSU BWTM5_MSU BWTM6_MSU BWWM6_MSU BWWM8_MSU BWWM9_MSU and BWWM16_MSU) were selected for studying on root colonization and plant growth promoting properties under aseptic condition. The results showed that BWTM5_MSU had the evident on the promotion of plant growth with the most percentage of rice seed germination (63.33%) high level of root and shoot length with 6.11 cm and 3.89 cm respectively. As a result the root and shoot fresh weight was as high as 9.56 mg and 11.33 mg respectively. It also correlated with the highest production of indole-3-acetic acid up to 421.29 ug/ml. The root colonization was studied by counting rhizobacteria isolate BWTM5_MSU from rice rhizoplane and rhizosphere with presented bacterial number at 9.67x106 CFU/g root and 1.64x10 10 CFU/g soil respectively.