The research team aimed to develop the cracking and extracting processes for Ganoderma lucidum (Red Lingzhi) spores and transfer the technology in the form of soap bar and liquid products together with the cracking and extraction process to the community at Farm Haid Klang Ban in Pathumthani Province. From the study since higher more bioactive compounds found in the aforementioned Ganoderma (Lingzhi) spore extracts over its basidiospore itself the optimized breaking and extraction procedure were composed of freezing at -20oC for 2 days bead beating for 5 minutes and then spore fermented with Lactobacillus spp. at room temperature for 24 hours. The processes could provide more than 65 percentages of the spore fracture efficiency and obtain more active substances such as polysaccharides and triterpenoids Moreover the spore extraction process was required by shaking in ethanol dissolved in distilled water at the ratio 80:20 for 2 hours. The obtained bioactive Lingzhi spore extracts as one active ingredient was added into the saponification process to make soap bar and liquid product. It was found that the soap products could create satisfaction and good feedback from high-level consumers of the farm community in Pathumthani Province. The research should therefore be successfully applied in the form of commercial red lingzhi extract soap in the manner of integration of science and community with knowledge of efficient Red Lingzhi spore cultivation and separation. It can help the development of community enterprises can bring up sustainable economic development in the country in the future.

The research team has isolated the cellulase producing microorganisms which have capability in producing extracellular cellulase enzyme for cellulosic degradation from leftover agricultural waste materials such as fruits and vegetables. After cellulosic material degradation the products mainly were sugar moiety being the starting materials for further alcohol fermentation and/or possibly being used in cultivation of common microorganisms. In the study when different 49 bacterial isolates were tested for their cellulase activity on Carboxymethyl cellulose (CMC) two bacteria Bacillus spp. CE08 and 49 were selected due to high cellulase activity and thus tested for the physical and chemical properties. Their 16S rDNA sequence comparisons were close to Bacillus sp. isolate obtained from the soil. Both isolates are common aerobic/facultative aerobic bacteria with the length of 1-3 μm Gram positive and preferring the fermentation of maltose and xylose to glucose while minimal degradation on sucrose and lactose contents. Their optimum digestibility was at 40°C pH 4.5 for the broth having unpurified extracellular cellulase activity at 3-4 μg /mg. The enzyme could use the substrateCMC the best but rice straw more slowly but better than grass. However the enzyme displayed the least digestion effectiveness on cotton. Both of these isolates are expectedly applied in the biological degradation process substituting chemicals that cause pollution problems to the environment. Additionally this is one promise way to bring value of agricultural waste to the community.

It is well known that waste spoiled are undesired and one of the main causing of environmental problems and health effects. In Thailand there are growing wastes especially at Thewarat market every year. The causes of the increase in wastes are from economic and industrial expansion which are important issues for the community responsible to manage and correct the amount and types of waste disposed properly before disposal. In the study a preliminary survey by talk and providing the questionnaires were conducted at the Thewarat market. It was found that solid wastes or rubbishes are mostly generated during and after trading process with and without proper can disposal. The stores did commonly dump the solid and liquid wastes around the stores or nearby the bin not inside the bin. The unpleasant observations shown were the solid wastes and dirty water contaminating into the soil and in the waterway and it was not managed hygienically. Uncared by unconcerned sellers during busy market during the day more unrecognized attitudes of the merchants themselves inappropriate placement of food and food waste in the public for the buyers and manucipal wastes generated by people in the surrounding community were normally observed daily. More risks of disease spreading and accumulated rodents insects and pathogens breeding and homing were appeared during at night after the market closed. In the research the campaign and suggestions were operated by the research team the proposed strategies for the waste management at Thewarat market area were including two times of training; discussions and 2 leaflets. These sessions were based on the education on proper waste management highlight on the proper use of right trash bin with the right waste post-sales area management and the use of microorganisms for the production of bio-fertilizers and bio-fermented solution from biodegradable waste in the market community. Moreover the monitoring communities were done for the proper use of trash bin in the community and providing the right waste management. It could bring the benefits to the community at the initial level especially the realization and attitudes in the suitable waste management since approximated 10% shops keep their shops and area nearby clean thus adding a panoramic view with a good image to the Thewarat market.

The study is the development of biological methods for extracting gold from the effluents of the gold processing and gold extraction in the industries where has a very high acidity (pH 0-4). This method should be used to replace the electrochemical process and chemical method which cause the pollution of toxic substances acid fumes and heavy metals of copper cyanide to the environments. The research started at the characterization and identification of microorganisms presented in gold contaminated water in natural source where is highly acidic (pH 2-4) and it could synthesize nanogold particles. The bacteria Bacillus sp. E201 were identified from this source and it showed its 16S rDNA gene with similarity of 95% to bacteria Bacillus thuringiensis accession no. FJ755916. It could create gold in the solution outside the cells within three days Form 1 mM HAuCl4 • 3H2O solution the bacterial dry weight could yield 75.3% with the amount of 0.5115 grams. It could be able to withstand acidic conditions and harvest gold from the gold extraction solution after centrifugation and filtration. So this developed biological method could be developed to apply in the industrial production of gold and jewelry industry.