Most of ethanol production processes are limited by low ethanol production rate and recyclable problem of ethanologenic organisms. In the present study Saccharomyces cerevisiae TISTR 5339 entrapped in an aqueous mixture of alginate and pretreated-bagasse was used for ethanol production of immobilization parameter of yeast cells consisting of amount of bagasse and alginate concentration on ethanol production were investigated. The results showed that increase in alginate concentration and the amount of bagasse can increase structural strength and reduce deterioration of bead. The optimal condition of immobilization was 1.0% (w/v) bagasse and 2.5% alginate. Ethanol yield (Yp/s) ethanol production rate (Qp) and efficiency of fermentation (Ey) obtained from the bagasse-alginate immobilized yeast cell was 3.39±0.72 g/l0.14±0.03 g/l/h and 70.98±14.08% respectively. The ultrastructure obtained from SEM illustrated that S. cerevisiae TISTR 5339 was well incorporated within the bagasse-alginate matrix. Moreover the bagasse-alginate immobilized yeast cells were reusable at least 5 times with Ey higher than 50% of 1st round of fermentation. Therefore the pretreated bagasse-alginate immobilized yeast cells is an alternatively interesting techniques with effiiciency for ethanol production in the future.

The research aims to investigate the possibilities of crushed oyster shell (OS) not have to go through the process of burning as a substitution cement for concrete blocks. The cement was replaced with crushed oyster shell not process burning (OS) at 0% 10203040 and 50% by the weight of the binder. After that the concrete blocks were tested to determine the compressive strength and moisture absorbed of the concrete blocks cured for 7 142856 and 90 days The result showed that the compressive strength of the concrete blocks which replaced cement with OS (at the proportion of 10% by the weight of the binder) at 28 days had the compressive strength and water absorbed accordance with the TIS 58-2533 on non-load bearing concrete blocks.Moreover the concrete blocks which replaced cement with OS at the proportion of 20 % and 30 % had the compressive strength equivalent to 28 days compressive strength of TIS 58-2533 standard at 56 and 90 days respectively and decreased when the percentage in addition to OS in concrete blocks of 40 and 50 %. Tested results also showed that the water absorbed of concrete blocks increased when the percentage of replacement of OS in binder increased at 10 20 30 40 and 50%. The economical of concrete blocks it has manufactured unit cost is 2.60 baht. Whereas the concrete block containing crushed oyster shell at 10-30% it has manufactured unit cost at 2.12-2.44 baht respectively.

This work presented the production of biogas using anaerobic co-digestion between hydrilla verticillata (HV) from Songkhla Lake Basin and chicken manure (CM). From the study of compositions of both wastes it was found that the COD of CM and HV were 11200 and 14400 mg/l and pH were 6.5 and 5.5 respectively. The biogas production using anaerobic single-digestion of untreated hydrilla verticillata (UHV) gave accumulative methane of 1251 ml-CH4/g-VS and methane production of 78.28 L-CH4/kg-substrate was obtained. For hydrilla treated with 2% w/v NaOH (THV) the accumulative methane was 1786 ml-CH4/g-VS and the obtained methane production was 91.87 L- CH4/kg-substrate which was 17.5 % increase when compared to the UHV. For the biogas production using anaerobic single-digestion of CM it was found that the accumulative methane and methane production were 978 ml-CH4/g-VS and 78.28 L- CH4/kgsubstrate respectively. These were lower than the studies using HV. The study of anaerobic co-digestion of HV and CM at three different ratios; 1:1 2:1 and 3:1 was studied. It was found that the anaerobic co-digestion using THV and CM at ratio 3:1 and 2:1 gave the highest potential for biogas production. The accumulate methane of 3202.20 and 2079.19 ml- CH4/g-VS were obtained respectively. The methane productions were 2.53 and 82.26 L-CH4/ton-substrate for ratio 3:1 and 2:1 respectively which is no significant different at 95 % confidence interval.

This research aimed to develop compound of prototypes polymer bio-composites from cassava starch (CS) reinforced with agriculture residue the rind of durian fruit. Cellulose extracted from durian rind using hot sodium hydroxide and bleaching treatment with hydrogen peroxide. The removal of lignin and hemicellulose components from durian rind were investigated by fourier transform infrared spectroscopy (FT-IR). Cassava starch bio-composite films were prepared by solution-casting technique which includes 0-50 wt% of durian rind (DR) or durian rind cellulose (DRC) as reinforcement agent and urea/glycerol (GU ratio = 50:50) as plasticizer. The physical and chemical properties of thermoplastic starch (TPS)/DR and TPS/DRC bio-composite films were investigated. The percent elongation at break of bio-composite films were increased when compared to control CS film. From 15TPS/DR (80/20) films showed increase the elongation at break from 7.28 to 14.00% when compared to CS control films. While 15TPS/DRC (80/20) bio-composite films exhibited that the highest elongation at break to 77%. Durian fiber (DR or DRC) loading decreased the water absorption of plasticized bio-composite films. Furthermore incorporation of DRC in composite can be enhanced film stability in water better than DR. Therefore the effect of DRC reinforcement agent on TPS bio-composite films can improve water resistance and mechanical properties especially in terms of flexibility. Finally the waste of bio-composite films was degraded completely in soil burial test.

The objectives of this research were to determine the suitable times of pork slices drying using microwave followed by hot air and to find specific energy consumption during drying. The physical characteristic (color and hardness) and the sensory test of dried pork slices were also investigated. Pork slices with the sizes of 50x50x1.3 and 50x50x2.2 millimeters were dried using the microwave at the powers of 160 320 480 640 and 800 watts until the samples did not burn during drying. Then the samples were dried using hot air at a temperature of 110oC until the moistures of the samples were lower than 20% dry basis. Based on the experimental results it was found that the suitable times of the samples drying using microwave were 50-600 seconds which depended on the microwave powers and the pork thicknesses. In hot air drying period drying times of the samples with thicknesses of 1.3 and 2.2 millimeters were 30 and 45 minutes respectively. The microwave powers had no significant effect on the redness (a value) and yellowness (b value) of dried pork the hardness of dried pork and the specific energy consumption (P<0.05). The pork thicknesses had the significant effect on the lightness (L value) and yellowness (b value) of dried pork the hardness of dried pork and the specific energy consumption (P<0.05). Moreover sensory scores of the dried pork obtained from this research were close to those obtained from the local market.