This experiment was conducted to study on single cell protein production from copra meal residue by Saccharomyces cerevisiae and Bacillus subtilis as animal feedstuffs. The experimental design was 4x3 factorial in CRD experiment with two factors were design to experiment. The first factor was comprised of fermented period (0 15 30 and 45 days). While the second factor B was comprised of microorganism type (Saccharomyces cerevisiae Bacillus subtilis and Saccharomyces cerevisiae combined with Bacillus subtilis). The results showed that interaction between factor A and B on pH (4.00-4.53) and percentage yield (29.21-33.80) were highly significantly different (P<0.01). However Incubation temperature were not related between factor A and B. Additional both factor have no effect to incubation temperature (P?0.05). For nutrients composition the group was fermented Saccharomyces cerevisiae combined with Bacillus subtilis showed higher Crude Protein than other treatments (P<0.01). Optimum of period at days 45 showed that crude protein 14.77 percent was significantly (P<0.01) and pepsin digestibility 66.60 percent was significantly (p<0.01)

The objectives of this research were to study the reduction of organic compounds by the biological action substance of the effluents from palm oil mil to produce singe cell protein. The wastewater taken from the third pond of the wastewater treatment system which was already used for biogas production was found to have pH 6.5. The organic matter like COD (3679 mg/L) Oil& grease (133 mg/L) as well as the total solids and the minerals were much lower than those of the decanter effluent. The reduction of organic compounds by using the biological action by Candida tropicalis TISTR 5146 5045 and C. lipolytica TISTR 5151 were studied. The results indicated that C. tropicalis TISTR 5146 which was cultivated in palm oil mill at initial pH 5.5 and diluted with water to the ratios of 1:5 at agitation on the shaker of 150 rpm at room temperature gave the highest yield of biomass of 2.52 g/L and COD reduction of 94.38 % after 3 days of cultivation. The optimal condition for C. tropicalis TISTR 5146 cultivation was that the ratio of palm oil mill effluent to diluted water was 1:5 containing 10 g/l glucose and 10 g/l yeast extract at initial medium pH 5.5 in the shaking flask of 150 rpm at room temperature. The highest biomass obtained was 12.53 g/L while the cell consisted of protein and fat for 43.12 % and 0.34 % respectively.