The study used the bark leaves and fruit of Ficus hispida Linn to separation by Maceration and sequential extraction method with hexane ethyl acetate and methanol to crude extracts. Crude extracts were assessed for their antioxidant activities tested using DPPH radical scavenging assay total phenolic contents tested using the Folin–Ciocalteu method cytotoxic activity against oral cavity cancer (KB) human small cell lung cancer (NCI-H187) and breast cancer (MCF-7) cancer cell lines tested using the resazurin microplate assay (REMA). The results showed that 1. Crude methanol extracts from the fruit exhibited free radical scavenging effect on the DPPH assay with IC50 value of 27 g/ml with the total phenolic contents value of 102.39 mg GAE/g dw of standard BHT. 2. Crude hexane extracts from the bark showed highest inhibited the growth of Vibrio paraheamolyticus crude ethyl acetate extracts from the bark showed highest inhibited the growth of Streptococcus mutans ATCC 27175 and crude methanol extracts from the leaves showed highest inhibited the growth of Vibrio cholerae most with MIC value of 7.80 g/ml. 3. Crude ethyl acetate and methanol extracts from F. Hispida exhibited inhibitory effect against oral cavity cancer (KB) human small cell lung cancer (NCI-H187) and breast cancer (MCF-7) cancer cell lines. These data support traditional uses of F. Hispida in folk medicine

A novel and simple method for measuring total antioxidant capacity or T AC in Thai herb extracts has been developed. The principle is based on generating free radical the deep blue Imipramine radical is generated by oxidizing Imipramine by ammonium tetravanadate in acid medium that can be easily scavenged by antioxidant compounds in the sample. Decreasing in the absorption spectrum at 620 nm related with antioxidant capacity was detected by spectrophotometer method in the proposed of practicable and low-cost However improvement of the throughput Flow injection analysis (FIA) was applied. The variables affecting the signal such as pH reagent concentrations linear relationship was optimized. The novel assay is linear up to 2800 mg Trolox equivalent/L its precision value is lower than 1% RSD and these are no interference from NaOH NaCI and N~C03 The method was applied for determination of three extract fractions of G. sootepensis. The results show the different of antioxidant capacity in each fraction with S I has the most TAC following S2 and S3 respectively. Although a good correlation of the present method was observed between two methods DPPH and Follin Ciocalteu reagent Finally the developed method was easy stable inexpensive environmental friendly and automated measures total antioxidant capacity.

To study the effect biological activity of a fig tree extract using Sequential Extraction and use of fennentation Maceration using solvent 3 types of hexane ethyl acid state and methanol to extract 12 Chanin made. tested oral cancer cells (human mouth carcinoma: KB) lung cancer cells (human small cell lung cancer: NCI-HI87) breast cancer (breast cancer: MCF-7) anti-malarial. (Anti-malaria; Plasmodium falciparum) Microculture Radioisotope Technique used to test anti-tuberculosis (Anti-Mycobacterium. tuberculosis. anti-TB) to the Green fluorescent protein microplate assay (GFPMA). The results showed that extracts of the fig tree has anti-cancer Including breast cancer lung cancer and oral cancer. Extracts of the fig tree does not have anti-tuberculosis and extracts of the fig tree shows some anti-malarial. In further research should study the molecular and biological activity of the fig tree from the roots seeds leaves flowers and fruit to the biological activity ofthe data for a selection of extracts from medicinal plants to make. Purification and further develop research in the next commercial.

The objective of this study were to use of RAPD technique for identification of Krachai ecotype in Thailand. DNA from 36 sample of Krachai that collected from every part of Thailand are amplified by 10 primer and relation analysis by UPMGA. This study was conducted at Faculty of Agriculture at Bangpra Sriracha Chonburi. The results showed every primer can made different pattern. After comparison of RAPD pattern from each sample can divide into 2 groups. However every sample is closely genetic relation likes it came from same original.