Premna trichostoma Miq. (Lamiaceae) mainly distribute in Asia including Thailand. Folk healers use this plant to treat fever as a juice splash and bathe. The aims of this study are to evaluate the quantity of active constituents and antioxidant activity of P. trichostoma leaf extract. Total phenolic compounds were determined using Folin-Ciocalteu method. Total flavonoid contents were determined by Aluminum chloride method. The amount of apigenin and kaempferol contents were analyzed by high performance liquid chromatography (HPLC). In addition the antioxidant activity was evaluated by DPPH and ABTS assay. The results showed that total phenolic contents total flavonoid contents and the amount of apigenin and kaempferol of P. trichostoma leaf extract was 46.45±15.30 mg GAE/g extract 25.48±2.02 mg CE/g extract 11.16±0.04 and 4.54±0.08 µg/g extract respectively. The IC50 values from the DPPH and ABTS assay was 117.50±1.22 and 36.14±1.53 µg/ml respectively. This study shows the antioxidant properties and active constituents of P. trichostoma leaf extract which indicates the potentials for further development of this plant

The aim of this research was determine total phenolic content total flavonoid content and antioxidant activity by DPPH and ABTS assay from leaves flower and stem of Crateva adansonii DC. subsp. trifoliate (Roxb) Jacobs that was extracted by Soxhlet method using ethanol dichloromethane and ethyl acetate as solvent. Leaves extract showed the highest total phenolic content and antioxidant activity. Ethanolic leaf extract showed the highest total phenolic content (157.13±0.32 mg GAE/g extract) and antioxidant activity by DPPH assay (53.64±3.78% (IC50 = 1.49±0.02 mg/ml)) and ABTS assay (76.51±0.97% (IC50 = 0.84±0.01 mg/ml)).When we considering solvent effect found that ethanolic extract showed the highest total phenolic content and antioxidant activity. Total flavonoid content ethyl acetate extract showed the highest total flavonoid content. Total phenolic content significantly correlated with antioxidant activity.

The fresh moonflower (FM) was dried in hot air oven at 50 C 48 hours as the dried moonflower (DM) 7.37 %. Then DM was extracted by ethanol concentration 95% and using the evaporator to evaporate ethanol solution. This study aimed to study the process which extract phenolic compounds (PC) from FM and formulate PC in pasteurized milk. From the results PC was 68.11±1.44 μg/ml. The final formula of pasteurized milk was added PC 1g /200 ml and was pasteurized at 72 °C 15 s. The (L*) 85.91±0.07 (b *) -1.92±0.06 (a *) 17.13±0.24 pH 6.58±0.00 total soluble solid 12 ˚Brix. It could be stored at 4 C for 7 days.