In this study 14 bacterial strains isolated from gastrointestinal tract of black tiger shrimp (Penaeus monodon) was investigated for their probiotic potential. The results showed that 6 strains namely S1 S3 S6 S7 T4 and T5 were able to degrade 3 nutrient substances including protein starch and lipid among which S3 S7 and S2 were the best tested nutrient-degrading bacteria respectively. Six bacterial strains were unable to lyse red blood cells including S2 S3 T0 T1 T2 and T3. S2 strain showed the strongest antibacterial activity against shrimp pathogenic Vibrio harveyi following an agar overlay technique. Based on environmental tolerance tests S2 and T0 strains grew well under wide ranges of environmental conditions e.g. sodium chloride concentration (0-8%) pH (6-10) and temperature (25-37C). As a consequence S2 strain had the most probiotic potential for shrimp culture owing to no hemolysis of red blood cells inhibition of V. harveyi growth protein and lipid degrading ability and high environmental tolerance for shrimp cultivation. The strain was identified as Micrococcus sp. S2. However additional study focused on immune response nutrient digestibility and growth promotion of the strain should be further performed in marine shrimp.

The amount of dissolved oxygen (DO) in aquaculture ponds is considered to be one of the most important aspects for the survival of fishes. The water in aquaculture ponds is stratified into distinct layers with different DO caused by differences in temperature. This temperature difference occurs when the surface layer of water heated by the sun becomes hotter than the lower water layer. Since the top layer is in contact with the air it contains the most DO. On the other hand the deepest layer is isolated from the air it contains the lowest amount of DO. This study proposed an aeration system called a Solar Circulating-Water Aeration System (SCWAS). The SCWAS uses solar thermal energy to create a natural water circulation. In other words it provides an upward current of water from the bottom to the pond surface and a downward current from the surface to the bottom. This circulation creates the movement of surface water and then increases the DO content of the surface water. This water circulation moves highly oxygenated surface water to the bottom layer forcing low oxygen water from the bottom up to the surface where it can absorb oxygen. To evaluate this design concept the numerical simulations of the system were conducted using ANSYS Fluent software. According to the simulation results the SCWAS can provide the natural water circulation. It was found that a flow rate of 2.24 L/s is obtained when the ratio between the draft tube inlet (r) the draft tube outlet (R) the base (L) is 1: 2: 3. In addition an SCWAS prototype was built and installed in a tilapia aquaculture system. A calibrated measuring system was installed to investigate the prototype performance. The experimental results indicate that the average DO values at the water surface and at the bottom of the pond increased by 1.45 and 1.40 times respectively compared with those of a pond without SCWAS. Economic analysis shows that the payback period of SCWAS is 4.58 years and its investment cost is 17.49% less than that of the paddle-wheel aerator.