Physic Nut (Jatropha curcas L.) was a narrow genetic based plant resulting in low productivity. Colchicine application was a mutation technique that could induce genetic variability for plant improvement. In this study the 24 hour soaked physic nut seeds and the 3 – day physic nut seedlings were treated with 0 0.05 0.15 and 0.20% of colchicine for 48 hours and there were soaked for 24 hours to induce polyploidy. The results showed that the survival rates were 67.15 and 93.93 % at the 0.05% of colchicine. DNA content in the survived seedlings was detected by flow cytometry. The number of tetraploid and mixoploid were increased in the 3-day seedlings treated with 0.05% of colchicine. The morphology characteristics at the first flowering stage were follows; the diploid had average height branch numbers spikelets per head ratio of male and female flowes leaf size (W x L) and stomata size (W x H) were 249.50 cm. 15.7 214.7 24.8 :1 14.8 x 13.2 cms and 7.88 x 50.25 micrometers respectively while the mutant had the average of 180.6 - 21.54 cm. 9.8 - 13.0 37.9 - 17.53 19.3 : 1 42.8 : 1 17.0 - 14.4 cms and 61.0 - 70.5 x 103.33 - 116.1 micrometers respectively. so The mutant had better characteristics but their fruits could not mature.

The preparation of silver fiber with electrospinning process was a principle to impulse the iron within the polymer solution. The polymer solution jetted onto the ground collector in nonwoven form when the electric charge was higher than the polymer surface tension. This research had 3 key factors consideration that were electric power supply polymer concentration and length between needle tip and aluminum ground collector. The electric voltage was 6 9 12 15 and 18 kilovolt the ratio of Ag : polymer solution (%wt) was 1:10 1:20 and 1:30 and the length between needle tip and aluminum foil collector was 6 7 8 9 and 10 centimeter respectively. The fibers obtained were investigated and compared to the fiber morphology by Scanning Electron Microscope and the anti-bacteria test via AATCC test method 100-1999 (Quantitative) and 147-1998 (Quality). The optimal electrospinning condition was 18 kilovolt the ratio of Ag : PVA (%wt) was 1:20 at length 7 centimeter. The fibers were continuous average diameter as 183.3 nanometer and available to against bacteria.