This research is to study the quality development of silk fibers by nanotechnology for the Silkworm farmer business groups of Surin Province. The research purposes are to study the efficiency and process of silkworm rearing using nanotechnology. To test the quality of silk fibers obtained from raising silkworms using nanotechnology. To compare of silkworm rearing results using nanotechnology and to transfer technology to improve silk quality with nanotechnology for silkworm farming business group in Surin Province. The results are as follows 1. The researchers designed the recording schedule which the party can record the changes easily of silk worm development during each day. This research allows the researchers to collect data from the farmer is more easily and finely into 4 groups of experiments with 3 nanotechnology formulas: 1. ZINC OXIDE 2. SILVER NANO and 3. ZINC OXIDE and SILVER NANO with 50:50 percentages each. The silk used in the experiment was “Nang Noi” which is popularly silkworm variety in every household in Surin province.When experimenting must clean the house and rinsing the container with DI WATER for aperiod of 7 months 2. This research is divided into 4 groups. Group 1: Silkworm feed normal methods without the use of nanotechnology. Group 2 silkworms feed with Nano substances such as ZINC OXIDE SILVER NANO ZINC OXIDE and SILVER NANO in 15 percentage concentration. Group 3 silkworms feed with Nano substances are ZINC OXIDE SILVER NANO ZINC OXIDE and SILVER NANO with 30 percentage concentration. Group 4 silkworms feed with Nano substances are ZINC OXIDE SILVER NANO ZINC OXIDE and SILVER NANO with 45 percentage concentration 3. The Results and comparison of silkworm rearing using nanotechnology the best formula feed is formula 1. When feeding Nano-sprayed on mulberry leaves using 3% 15% zinc silver and silver zinc it is found that the growth of worms in each age was good growth and not being a disease. Moreover Silkworms are slightly better and more alert than normal farming. The length in ages 3 - 4 - 5 is going in a better direction. Amount of eating mulberry leaves in each age. As usually eating more in ages 2 and 3 after passing the silk production process and testing the quality of silk fibers in the laboratory were found that the silk fibers are smooth not lacking toughness: Tenacity at 3.06 4.60 and 4.60 respectively and also result in fiber amount increase and also the color of the silk fibers is golden quite clear beautiful and consistent color. 4. When technology transfer to the Silkworm farmer business groups of Surin Province as workshop for 3 days. Most participants are satisfied with the highest levels in all questions.

The objective of this research is to propose a new way to fabricate a composite material for future bone replacement development via growing apatite crystal on silk fibroin fiber. Apatite crystallization on silk fibroin fiber was prepared by precipitation reaction between Ca(NO3)2 solution and (NH4)2HPO4 solution adjusted pH by NH4OH solution. In this work Effect of solution concentration and initial pH values was investigated on apatite crystals. Chicken eggshells were used as calcium source by heating sufficiently powdered eggshells at 1300 °C for 4 h to acquire CaO powders made Ca(NO3)2 solution by dissolving in 65% HNO3 solution. Prepared Ca(NO3)2 solutions were adjusted concentration as 3 levels and were adjusted different pH values of 7 8 and 9 respectively. For processing of apatite crystallization silk fibroin fibers were immersed in Ca(NO3)2 solutions for 30 min. After that (NH4)2HPO4 solutions were added in silk fibroin fibers immersed in Ca(NO3)2 solutions. Reaction time was fixed about 1 min before cleaning to remove impurity precipitates on surface of silk fibroin fiber. Finally apatite crystallization on silk fibroin fibers occurred. The obtained result can conclude morphologies of apatite crystals on silk fibroin fibers was sheets sphere and flower. Solution concentrations and initial pH values directly influenced on Ca/P ratio of obtained apatite crystals by Ca/P ratio increases as increased solution concentrations or increased pH values. Finally research silk fibroin fibers were woven as two patterns of plain weave and twill weave and they were investigated Young’s modulus and tensile strength. The result indicates that Young’s modulus of silk fibroin fiber sheets woven as twill weave and as plain weave were about 616 MPa and 425 Mpa and tensile strengths of them weave were about 40 Mpa and 72 Mpa respectively. The optimum condition of growing apatite crystals on silk fibroin fibers was sample of CP19. Design of silk fibroin fiber with plain weave was an optimum pattern for future biomedical applicaions.