The objective of this study is to obtain a model that maximize production of γ-linolenic acid (GLA) by the moss Physcomitrella patens employing response surface methodology (RSM). The RSM with a three-variables (sucrose potassium nitrate and glutamate concentrations) and five-level (-2 -1 0 1 and 2) central composite design (CCD) including 20 experimental runs was employed to optimize the medium composition. Results showed that the experimental data could be appropriately fitted into a second-order polynomial model with a coefficient of determination (R2) more than 0 .9 5 for GLA production. Analysis of variance (ANOVA) revealed that the model was highly significant (p<0.0001) and the effects of the sucrose (20-100 g/L) and glutamate (0.5-2.5 g/L) concentrations on GLA production were significant (p<0.05). The GLA production with the optimized culture medium (sucrose concentration of 62.92 g/L potassium nitrate of 0.80 g/L and glutamate concentration of 1 .4 2 g/L) by RSM increased 4.61 folds when compared with the standard BCD medium. This experimental value (16.37 mg GLA/L) fit well with the predicted values (16.20 mg GLA/L).

Natural compost or organic fertilizers suitably applied to soil since it improves soil quality is cheap and easy to manage and has no pollution residues. However the right ratio of ingredienttypes as well as enough fermentation period have to be tested in order to obtain suitable fertilizer for each particular plant species. In this study researchers have conducted experimental fermentation of organic composts composed of various ratios of either residues of bagasse or banana pulp mixed with sludge. This organic compost performs as organic fertilizer adding nutrients to the sludge since the sewage sludge by itself can only help improve soil structure but has insufficient nutrients for the growth of plants. The sludge in the experiment was obtained from sewage sludge at WatSuanKaew Nonthaburi province. The results of the organic compost after fermented 49 days showed that the compost turned to brown and black and most plant materials were already macerated. The compost of sewage sludge mixed with banana pulp in the ratio of both 0.75 to 5.0 and 1.0 9 to 5.0 yielded the best growth of the morning glory. Both formulas accelerated the growth of seedlings of the plant to a high average of 15.0 ± 0.3 cm after grown for14 days. The concentration of main nutrients was also close to the values specified by the standard compost. This banana pulp compost with pH 7-9 helpedreduce the acidity in the soil. While organic fertilizer derived from sewage sludge mixed with bagasse could only help accelerate the growth of the seedlings but did not accelerate the proliferation of immature plants. Additionally the ratios of carbon to nitrogen in all bagasse composting samples were more inflated whereas the nitrogen phosphorus and potassium were under the specified standard. This bagasse caused lower pH to 5.0-6.0 contributing more acidic to soil. From all results of these findings the organic compost from the fermentation of banana leafs was therefore appropriated for improving soil quality by mixing with sludge. The organic compost provided enough major and minor nutrients that stimulated the plant growth as well. However the formulated ratio of the bananas leafs and sludge mixtures needed to be prior experimentally tested.

The objective of this study was to determine the effects of malate level and yeast in concentrate on ruminal fermentation and digestibility of nutrients in heifers. The results revealed that rumen fermentation ruminal microorganisms and digestibility of nutrients were significantly different (P<0.05) as affected by malate level and yeast. In conclusion the combined use of concentrate containing high level of cassava chip at 70%DM with malate at 1000 g and yeast at 2000 g in concentrate could improve rumen fermentation and digestibility of nutrients in dairy heifers.

In this study 14 bacterial strains isolated from gastrointestinal tract of black tiger shrimp (Penaeus monodon) was investigated for their probiotic potential. The results showed that 6 strains namely S1 S3 S6 S7 T4 and T5 were able to degrade 3 nutrient substances including protein starch and lipid among which S3 S7 and S2 were the best tested nutrient-degrading bacteria respectively. Six bacterial strains were unable to lyse red blood cells including S2 S3 T0 T1 T2 and T3. S2 strain showed the strongest antibacterial activity against shrimp pathogenic Vibrio harveyi following an agar overlay technique. Based on environmental tolerance tests S2 and T0 strains grew well under wide ranges of environmental conditions e.g. sodium chloride concentration (0-8%) pH (6-10) and temperature (25-37C). As a consequence S2 strain had the most probiotic potential for shrimp culture owing to no hemolysis of red blood cells inhibition of V. harveyi growth protein and lipid degrading ability and high environmental tolerance for shrimp cultivation. The strain was identified as Micrococcus sp. S2. However additional study focused on immune response nutrient digestibility and growth promotion of the strain should be further performed in marine shrimp.

Arachis glabata is new variety forage which tolerates drought and provide high protein.This kind of plant is suitable as dairy cow diet. The research was divided into 2 experiments Experiment 1 was conducted to study on nutritive value of Arachis glabata.The experimental design was Randomized Completely Block Design (RCBD) experiment with three treatments. Each treatment consisted of three blocks (three crops) and followed different three variety of Arachis glabata as Cv. Florigraze Cv. Arbook and Cv. Ecotart. Results showed that three variety of Arachis glabata were 28.26-30.75 % of dry matter (P>0.05). In addition Arachis glabata Cv. Arbook was grater 19.78 % of protein than other group of Arachis glabata (P<0.01). Furthermore Arachis glabata Cv. Florigraze was grater 17.63 of hemicelluloses and 3830.00 kcal/kg of gross energy significant different among other group of Arachis glabata (P<0.05). Experiment 2 was conducted to study on ruminal degradability of Arachis glabata using In situ technique in dairy cattle. The experimental design was Randomized Completely Block Design (RCBD) experiment with three treatments. Each treatment consisted of three blocks (three ruminally fistulated dairy cattle) and followed different three variety of Arachis glabata as Cv. Florigraze Cv. Arbook and Cv. Ecotart. Results showed that Arachis glabata Cv. Florigraze and Arachis glabata Cv. Arbook was grater dry matter organic matter and protein degradability at 0-4 hour and 24-28 hour than Arachis glabata Cv Ecotart (P<0.05). In addition kinetic of degradation (a b c and p) of dry matter organic matter and protein were not significant different (P>0.05) among three variety of Arachis glabata.