The aim of this study was to investigate the microbiological quality of milk-shaked drinks including Bacillus cereus Escherichia coli Salmonella spp. Staphylococcus aureus yeast and molds in unsealed containers sold in market fair roadside and stall around Mahasarakham university. The analytical results revealed that microbiological quality of 30 samples of milk-shaked drinks in unsealed containers that did not pass the criteria of department of medicine ministry of public health Thailand were 50 % of total samples. Bacteriological counts of Bacillus cereus Escherichia coli Salmonella spp. And Staphylococcus aureus of milk-based drinks that did not pass the criteria were 13.3 10 13.3 and 10% of total samples respectively. Yeasts and molds counts of milk-based drinks that did not pass the criteria were 3.3 and 30% of total samples respectively. Microbiological contamination of milk-shaked drinks was related to the hygiene of the shop and the supplier. It was found that the location of stall which high dust particles and animal carriers also contributed to the contamination of microorganisms. Moreover the hygiene of the supplier may cause the milk-shaked drinks to be contaminated with microorganisms.

Using of ethylenediaminetetraacetic acid (EDTA) and synthetic surfactant increase the chemical contamination in water. The co-phytotoxicity of EDTA (0-20 mg/l) and 3 synthetic surfactants; Triton X-100 (0-10xCMC) Tween 80 (0-10xCMC) and sodium dodecyl sulfate (SDS) (0-2 x CMC) on the growth of 2 duckweeds spirodela polyrrhiza and Lemma perpusilla were studied for 3 days. The results showed that the surfactant were phytotoxic on the growth of both plants while EDTA was not. SDS was the most toxic to plant especially on all chlorophyll content. Triton X-100 was only toxic to the growth of L. perpusilla and weight of S. polyrrhiza. Tween 80 was less or the least toxic to both plants. This surfactant only decreased chlorophyll a and total chlorophyll content of S.polyrrhiza and L. perpusilla. There was not any interaction between EDTA and surfactant on most toxicity tests. With these results synthetic surfactant contamination should be more concerned than co-contamination with EDTA.

The purposes of this research were to assess the combined phytotoxicity of glyphosate and carbendazim which were normally used as herbicide and fungicide contaminated in soil as 100 – 1000 mg/kg on seedling growth of ridge gourd. Both of glyphosate and carbendazim were toxic to shoot and root growth of ridge gourd seedlings. The 50% effective concentration (EC50) on shoot length root length root fresh weight and shoot dried weight of ridge gourd seedlings for glyphosate were 669.4 413.0 574.5 and 241.4 mg/kg respectively whereas for carbendazim were >1000 357.8 >1000 and 71.4 mg/kg respectively. Co-contamination between glyphosate and carbendazim seemed to be antagonistic effect. Both of glyphosate and carbendazim at all concentration were not toxic to chlorophyll content in cotyledon of ridge gourd seedlings.

Use of synthetic surfactant is another choice to increase efficiency of polycyclic aromatic hydrocarbon (PAH) phytoremediation in soil. In this study the toxicity of soil contaminated with anthracene and fluoranthene at total concentration as 0 200 and 800 mg/kg in combination with 2 synthetic surfactants that were triton x-100 and tween 80 at concentration as 0 1 and 10 times of critical micelle concentration (CMC) on seedling growth of Hibiscus sabdariffa and Mirabilis jalapa. Both synthetic surfactants were watered separately on day 13 after inoculation and seedling growth were measured on day 20 after inoculation. The result shown that both H. sabdariffa and M. jalapa could grow in soil contaminated with anthracene and fluoranthene at all concentrations in combination with both synthetic surfactants. Tween 80 at 1 time of CMC were the least toxic to H. sabdariffa while toxicity of all surfactants on M. jalapa were not different. Decrease of plant growth depended on the increasing of PAH concentration in soil. With these reasons both plants were possible to use for PAH phytoremediation at total concentration 200 mg/kg in combination with synthetic surfactant