In this research scaffolds of β-tricalcium phosphate (β-TCP) and zinc nickel ferrite (ZnxNi1-xFe2O4) were simply fabricated via the uniaxial pressing together with pressureless sintering which can be tailored for specific applications. The morphology of ZnxNi1-xFe2O4/β-TCP scaffolds with all formulas obtained from the SEM result revealed the larger grains the higher porosity and higher interconnectivity with increasing of ZnxNi1-xFe2O4 amount in β-TCP matrix. The high porous structure of the composite scaffold provided high surface area for cell attachment indicative of good cell growth and differentiation. Biological in vitro test using direct contact method with human cells revealed that the composite scaffold of β-TCP and Zn0.8Ni0.2Fe2O4 with all ratios induced the higher growth of Human osteoblast cells and the better cell spreading on surface of specimens

This study was conducted to determine the effects of plant growth regulators on in vitro propagated plantlets and microrhizome inductions of Curcuma comosa Roxb. for an efficient development of plant regeneration. In vitro sprouted shoots (2 cm length) of C. comosa were incised in longitudinal section and subcultured on MS media supplemented with 3 mg/L BA for shoot multiplication and plant regeneration. Subsequently the regenerated plants were transferred to MS medium with various concentrations of plant growth regulators; BA (1 3 5 mg/L) kinetin (1 3 5 mg/L) and TDZ (0.5 1 1.5 mg/L). The result showed that MS medium supplemented with 3 mg/L BA was the most effective in shoot induction with the significantly maximum average number of 2.11 shoot buds per responding explant. Moreover MS medium supplemented with 1 mg/L BA produced the longest average of shoot bud explants (12.99 cm) while the basal MS medium induced the longest average of root multiplication (6.63 cm). Furthermore microrhizome induction was produced under in vitro conditions derived young shoot buds upon transfer to MS medium containing various combinations of 1 3 and 5 mg/L BA and 30 60 and 90 g/L sucrose concentrations. The optimum combination in the induction of significantly highest average length of both stem (18.32 cm) and root (7.52 cm) multiplication was obtained on MS basal medium supplemented with 1 mg/L BA and 30 g/L sucrose for 4 weeks. Although microrhizome formation was not produced by the different amount of BA and sucrose in induction medium starch accumulation was found at the base of the stem explants cultured on MS medium containing 60 and 90 g/L sucrose concentrations. Additionally in vitro propagated C. comosa plantlets through microrhizome induction were grown under greenhouse conditions for 4 weeks and further developed into normal plants resulting in successfully high survival rates of 66 – 100% in the cultivated C. comosa.