The research team has isolated the cellulase producing microorganisms which have capability in producing extracellular cellulase enzyme for cellulosic degradation from leftover agricultural waste materials such as fruits and vegetables. After cellulosic material degradation the products mainly were sugar moiety being the starting materials for further alcohol fermentation and/or possibly being used in cultivation of common microorganisms. In the study when different 49 bacterial isolates were tested for their cellulase activity on Carboxymethyl cellulose (CMC) two bacteria Bacillus spp. CE08 and 49 were selected due to high cellulase activity and thus tested for the physical and chemical properties. Their 16S rDNA sequence comparisons were close to Bacillus sp. isolate obtained from the soil. Both isolates are common aerobic/facultative aerobic bacteria with the length of 1-3 μm Gram positive and preferring the fermentation of maltose and xylose to glucose while minimal degradation on sucrose and lactose contents. Their optimum digestibility was at 40°C pH 4.5 for the broth having unpurified extracellular cellulase activity at 3-4 μg /mg. The enzyme could use the substrateCMC the best but rice straw more slowly but better than grass. However the enzyme displayed the least digestion effectiveness on cotton. Both of these isolates are expectedly applied in the biological degradation process substituting chemicals that cause pollution problems to the environment. Additionally this is one promise way to bring value of agricultural waste to the community.

Extraction of plastic and aluminum foil from beverage cartons with biological processes is one environmentally friendly approach that is appropriated for paper recycling. The other remained parts including plastic and foils can thus be utilized. This research aimed to improve the capability in digestion of cellulose paper of microbes isolated and screen from fruit durian and jackfruit in degradation of beverage cartons. In the small scale processes reducing sugar indicating the efficiency of the degradation process of the boxes was released from the hydrolysis of paper and determined by biochemical method of Benedict test. The controls of alkali and water immersion were utilized for these experiments. In the medium and large scale digestion processes the results showed that microorganisms helped separate sheet papers from the plastic and aluminum foils shortly in 14 days after boiling in water for 10 min. The bacteria digestion were well adapted within 14 days and prolonged their high degradability until 28 days. They could degrade the cellulose components better than the fungi with the separation efficiency of 70 percent and 66 percent respectively. Therefore from overall the biodegradation process of microorganisms have a high potential in digestion of various paper products for recycling and can be applied in industrial applications.