In this work fiber mat based on silk sericin and thunbergia laurifolia linn extract was developed via electrospinning technique for wound dressing application. The waste cocoons were selected from Nakhon Ratchasima 1 (K1) with appropriate spicy for extracting the silk sericin protein. Trunk of thunbergia laurifolia linn was selected for preparing thunbergia laurifolia linn extract. Polyvinyl alcohol (PVA) with several concentrations were chose and fabricated to fibers by electrospinning technique to investigate suitable polymer concentration for mixing silk sericin and thunbergia laurifolia linn extract. The result revealed that PVA of 9 wt % was suitable due to Several parameters were investigated to prepare the electrospun fiber of silk sericin and thunbergia laurifolia linn extract such as their mixing ratio voltage the distance between the needle and the substrate and the needle size. The results indicated that mixing ratio of silk sericin and thunbergia laurifolia linn extract at 50:50 (w/w) voltage of 15 kV the distance of the needle and the substrate at 8 cm and the needle size of 18 were optimum condition for the fabrication of electrospun fiber with diameter of 100-200 nm and that with smooth and continue feature.

Degumming process is a fundamental finishing process for silk yarn and silk fabric. The main objective is scouring the substrate such as silk gum (sericin) wax and some impurities from silk fiber. The principle of degumming process is breaking the peptide linkage of amino acid in sericin structure into a small molecule which is soluble in water. The hydrolysis reaction performed by acid and alkaline but they have a big problem on the surface area of silk. Proteolytic enzyme be used to solve this problem but it has some disvantages such as it was using a specific condition and expensive. For this reason this research chooses papain enzyme form dried latex of Carica payapa Linn. to degum the raw silk. The efficiency of degumming process was evaluated by determination of tensile strength and staining test with direct dyes (C.I. Direct Red 80). The result was revealed; the appropriate conditions for silk degumming with dried Carica payapa Linn.’s latex be recommended as follows: the amount of dried latex solution of 4 % owf at 75 degree Celsius for 30 minutes in this condition was not harm to strength and fiber surface. The degummed fibers still had lustrous soft and smooth surface.