The purposes of the study on nutrition knowledge of primary school students of Anuban Khon Kaen School Khon Kaen were to 1) study the nutrition knowledge of primary school students and 2) compare their nutrition knowledge. The samples were 363 Prathom Suksa 4 to 6 students in Khon Kaen kindergarten sampled by multi-stage sampling. The instruments used were four-choice quizzes with 30 question items. The reliability of the questionnaire was .8968. The collected data were analyzed by frequency percentage mean independent sample t-test ANOVA F-test and Scheffes method. The study revealed as follows. 1) Nutrition knowledge of primary school students at Anuban Khon Kaen School Khon Kaen in the overall was at the highest level. 2) The difference of nutrition knowledge between boy and girl primary school students at Anuban Khon Kaen School Khon Kaen was statistically significant at the .05 level. 3) Prathom Suksa 4 students and Prathomsuksa 5 students had a statistically significant difference in nutrition knowledge at the .05 level. Prathom Suksa 4 and Prathom Suksa 6 students were different in nutrition knowledge with statistically significant at the .05 level.