จำนวน Repository ( 6 )
การวิเคราะห์ปริมาณสารสำคัญและฤทธิ์ต้านอนุมูลอิสระของสารสกัดใบกะเปียดเครือ
Premna trichostoma Miq. (Lamiaceae) mainly distribute in Asia including Thailand. Folk healers use this plant to treat fever as a juice splash and bathe. The aims of this study are to evaluate the quantity of active constituents and antioxidant activity of P. trichostoma leaf extract. Total phenolic compounds were determined using Folin-Ciocalteu method. Total flavonoid contents were determined by Aluminum chloride method. The amount of apigenin and kaempferol contents were analyzed by high performance liquid chromatography (HPLC). In addition the antioxidant activity was evaluated by DPPH and ABTS assay. The results showed that total phenolic contents total flavonoid contents and the amount of apigenin and kaempferol of P. trichostoma leaf extract was 46.45±15.30 mg GAE/g extract 25.48±2.02 mg CE/g extract 11.16±0.04 and 4.54±0.08 µg/g extract respectively. The IC50 values from the DPPH and ABTS assay was 117.50±1.22 and 36.14±1.53 µg/ml respectively. This study shows the antioxidant properties and active constituents of P. trichostoma leaf extract which indicates the potentials for further development of this plant
ฤทธิ์ต้านอนุมูลอิสระและปริมาณสารประกอบโพลีฟีนอลในผลิตภัณฑ์อาหารว่างเสริมผงใบหม่อน
The potential health benefits attributed to mulberry leaf and its bioactive compounds have led to a huge increase of mulberry leaf products in the food market. The objective of this research was to determine total antioxidant activities ( FRAP and DPPH assays) and polyphenol compounds in 4 snack products i.e. loaf-bread puifai (Thai steamed cupcakes) peakpoon (Green coconut sweet pudding) and snow skin mooncake fortified with 10% mulberry leaf powder from “ Buri Rum 60” cultivar. The result showed that antioxidant activities and polyphenol contents increased in 4 products fortified with mulberry leaf powder. Ferulic acid was the only phenolic compound found in all original products. Kaempferol quercetin and caffeic acid were significantly increased whereas ferulic acid was reduced in the modified formulas. Therefore these bioactive compounds properties of mulberry leaf powder suggested their possible use for fortifying the healthy foods.
ฤทธิ์ต้านอนุมูลอิสระของกุ่มบก
The aim of this research was determine total phenolic content total flavonoid content and antioxidant activity by DPPH and ABTS assay from leaves flower and stem of Crateva adansonii DC. subsp. trifoliate (Roxb) Jacobs that was extracted by Soxhlet method using ethanol dichloromethane and ethyl acetate as solvent. Leaves extract showed the highest total phenolic content and antioxidant activity. Ethanolic leaf extract showed the highest total phenolic content (157.13±0.32 mg GAE/g extract) and antioxidant activity by DPPH assay (53.64±3.78% (IC50 = 1.49±0.02 mg/ml)) and ABTS assay (76.51±0.97% (IC50 = 0.84±0.01 mg/ml)).When we considering solvent effect found that ethanolic extract showed the highest total phenolic content and antioxidant activity. Total flavonoid content ethyl acetate extract showed the highest total flavonoid content. Total phenolic content significantly correlated with antioxidant activity.
ฤทธิ์ต้านอนุมูลอิสระของสารสกัดจากมะเดื่อ
The study used the fruit of Ficus hispida Linn to separation by maceration and sequential extraction method with hexane ethyl acetate and methanol to crude extracts. Crude extracts were assessed for their antioxidant activities tested using DPPH radical scavenging assay total phenolic contents tested using the Folin–Ciocalteu method The results showed that 1. Phytochemical examination of the extracts of F. hispida alkaloids condensed tannins phenolic compounds triterpenes steroids cardiac glycosides anthraquinones 2. Hexane Ethylacetate and Methanol extracts have the ability to reduce the amount of DPPH. The EC50 values were> 1000 285 and 30 g/ml respectively. 3. Hexane Ethylacetate and Methanol extracts contained phenolic compounds values of 3.09 55.25 and 99.59 mg GAE/g dw respectively.
การศึกษาอุณหภูมิการอบแห้งรกสุกรและการสกัดโปรตีนและฤทธิ์ต้านอนุมูลอิสระ
This research aims to study the appropriate drying condition and extraction solvent for extracting protein and antioxidants from the porcine placenta. Three levels of drying temperature were used in this study which were 50 60 and 70ºC the result shows that the best drying condition for porcine placenta was 70ºC for 4 hours because this condition provided the least drying time and the lowest moisture content of porcine placenta (3.80±3.36% dry basis). After that the fresh and dried porcine placenta were extracted by using three solvents which were deionized water (DI) phosphate buffered saline (PBS) and hexane. The efficiency of extraction was investigated by measuring the crude protein and antioxidant of the porcine placenta. It was found that there was no protein and antioxidant in fresh and dried porcine placenta extracted by hexane. However the protein was found as 0.05±0.01 and 0.04±0.01 mg of bovine serum albumin/100 mg fresh for fresh porcine placenta and 0.12±0.030 and 0.07±0.016 mg of bovine serum albumin/100 mg fresh for dried porcine placenta extracted by DI and PBS respectively. In additional the antioxidant was found as 1.13±0.03 and 1.29±0.04 mg of trolox/100 mg fresh for fresh porcine placenta and 3.37±0.02 and 3.59±0.01 mg of trolox/100 mg fresh for dried porcine placenta extracted using DI and PBS respectively.