จำนวน Repository ( 19 )

ผลของไคโตซานต่อปริมาณสารต้านอนุมูลอิสระและการต้านทานโรคใบไหม้ในข้าวสังข์หยดพัทลุง
ผลของไคโตซานต่อปริมาณสารต้านอนุมูลอิสระและการต้านทานโรคใบไหม้ในข้าวสังข์หยดพัทลุง

This research was aimed to study the effect of chitosan on total polyphenol contents anthocyanin contents antiradical properties and resistance of rice blast disease in Sung Yod Phatthalung rice (Oryza sativar L.) cultivars. The chitosan was applied on rice leaves at concentrations of 50 100 and 500 ppm compared with the control (without the chitosan and only adding a 0.025% acetic acid respectively). The result showed that 500 ppm of chitosan could significantly increase the total polyphenol contents (220.75 mg GAE/100 g dry weight sample) anthocyanin contents (2.98 mg/100 g dry weight sample) and antiradical properties (83.15%) in the grain extracts of Sung Yod Phatthalung rice. In addition Necrosis test on rice leaves treated with 500 ppm of chitosans clearly indicated the plant resistance againt Pyricularia grisea. Therefore the future studies of chitosan activation mechanism will give more valuable information that possible to be applied in the healthy rice product development and fungal disease control.

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Antioxidant and  -glucosidase inhibitory activities from local vegetables in phatthalung province
Antioxidant and -glucosidase inhibitory activities from local vegetables in phatthalung province

The objective of this research was to evaluate the antioxidant and glucosidase inhibitory activities of 9 fresh and dried local vegetables extract from Kong Ra District Phatthalung Province such as Barringtonia acutangula (L.) Gaertn. Archidendron jirinaga Nielsen Scientific name: in process (Neang-rok: local name) Oroxylum indicum (L.) Kurz Ficus semicordata J.E.Smith Ficus oligodon Mig Ficus sp. Ficus obpyramidata King and Ficus fistulosa Reinw. The antioxidant activities were determined by DPPH radical scavenging. Results indicated that dried crude extract of Oroxylum indicum (L.) Kurz exhibited the highest DPPH radical scavenging activity at the final concentration of 5 mg/ml revealed the DPPH activity as 92.25%. Moreover the extracts were studied for their glucosidase inhibitory activities compare with authentic drug Acarbose Results indicated that dried crude extract of Ficus sp. exhibited the highest inhibitory activity revealed the inhibition activity as 85.46%. Thus these local vegetable might be possible new sources of glucosidase inhibition and antioxidants so there are suitable for use as functional food in the future.

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Efficiency of feronia limonia swingle branch crude extract asantioxidant
Efficiency of feronia limonia swingle branch crude extract asantioxidant

The objective of this research were to focus the antioxidant activity and separated the chemical compound of crude extract from Feronia limonia Swingle branch. After that the optimum antioxidant of crude extract was used to prepare the antioxidant lotion. The result showed that crude extract had total phenolic and total tannin contents 41.99 and 52.35 g/ml ; respectively and also had antioxidant activity (IC50) 12.55 ?g/ml Whereas BHT and BHA had IC50 8.96 and 9.61 ?g/ml. The separation of crude extract can be separated into four groups (F1 F2 F3 F4) and the results showed that F2 has the strongest activity against the others with IC50 = 9.53 ?g/ml. Then F2 was analyzed with GC-MS and found that the structure were Hexadecanoic acid Ethyl ester Trimethylsilyl ester and Dioctyl ester. The lotion was odorless white color with no phase separation with pH 8.0

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A test kit for measuring total antioxidant capacity
A test kit for measuring total antioxidant capacity

According to the studying this research absorbance detection system for flow injection analysis system was constructed by using syringe pump so the problem of bubbles in the system was solved successfully. The principle of the test-kit based on pushing the solutions into the system using double two ml syringe pumps. The solutions were mixed at mixing coil then the solution of the sample was inserted automatically by using six-port-valve. The detector was built from light-emitting-diodes (LEDs) with the wavelength at 620 nm. The product solution in cuvette was exposure then the light energy was transferred to electric current by photo-diode. The electricity was changed to voltage to micro-controller. The absorbance was calculated. The results absorbance and flow rate were controlled by utilization of Labview software. The absorbance of the samples from the developed system was compared to the commercial spectrophotometer. The mean absolute percentage error of the flow rate was 1.2%.

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A novel method for measuring total antioxidant capacity and its application to monitoring the antioxidant status in Thai herbs.
A novel method for measuring total antioxidant capacity and its application to monitoring the antioxidant status in Thai herbs.

A novel and simple method for measuring total antioxidant capacity or T AC in Thai herb extracts has been developed. The principle is based on generating free radical the deep blue Imipramine radical is generated by oxidizing Imipramine by ammonium tetravanadate in acid medium that can be easily scavenged by antioxidant compounds in the sample. Decreasing in the absorption spectrum at 620 nm related with antioxidant capacity was detected by spectrophotometer method in the proposed of practicable and low-cost However improvement of the throughput Flow injection analysis (FIA) was applied. The variables affecting the signal such as pH reagent concentrations linear relationship was optimized. The novel assay is linear up to 2800 mg Trolox equivalent/L its precision value is lower than 1% RSD and these are no interference from NaOH NaCI and N~C03 The method was applied for determination of three extract fractions of G. sootepensis. The results show the different of antioxidant capacity in each fraction with S I has the most TAC following S2 and S3 respectively. Although a good correlation of the present method was observed between two methods DPPH and Follin Ciocalteu reagent Finally the developed method was easy stable inexpensive environmental friendly and automated measures total antioxidant capacity.

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