Nitrogen is an essential nutrient for plant growth. When the plants have been grown for a long-term effect to decreased nitrogen in soil and insufficiency of the plant growth. One biological method for increasing the nitrogen content in soil is utilize of free nitrogen-fixing bacteria Azotobacter. Therefore the objective of this research was to search natural material suitable for Azotobacter fixation. The 10 natural materials were observed physical structure and investigated the ability of natural materials for Azotobacter fixation. The results showed that water hyacinth and bagasse stalks were fixed the Azotobacter of 2.43x108 and 1.94 x108 CFU per gram of each natural material respectively. After that the selected fixed Azotobacter of water hyacinth and bagasse stalks were studied the growth of Brassica Chinensis. This experiment was designed to 4 treatments. The first treatment was mixed of water hyacinth stalks and the Azotobacter. Second treatment was mixed of bagasse stalks and the Azotobacter. The third treatment was the Azotobacter only. The last treatment was soil only. The results indicated that the average of plant growth include height leaf number root length fresh weight and dry weight of the mixed of water hyacinth stalks and the Azotobacter had significantly higher growth rates than other treatments. The total nitrogen was also increased 1.4 fold in the soil. All results suggest that the water hyacinth stalk is alternative for the fixation of the free nitrogen-fixing bacteria Azotobacter as induce nitrogen in the soil and plant growth promotion.

This research aims to study phytochemicals and potential of Mapok (Parinari anamense Hance) extracts to inhibit germination and growth of Barnyard grass (Echinochloa crus-galli) a common weed in the rice fields. Organic solvents extracted the part of fruits barks and leaves of Mapok included hexane ethyl acetate and methanol. Crude extracts compose of fruit hexane extract fruit ethyl acetate extract fruit methanol extract bark hexane extract bark ethyl acetate extract bark methanol extract leaves hexane extract leaves ethyl acetate extract and leave methanol extract. Crude extracts were tested of phytochemical tested of germinating inhibition root length inhibition stem length inhibition and dry weight inhibition of Barnyard grass. Leaves ethyl acetate extract showed highest inhibit germination and growth of Barnyard grass The leave ethyl acetate extract was separated by column chromatography to yield nine fractions (F1 – F9 fraction) and tested inhibit germination and growth repeating. The results showed that 1. The crude ethyl acetate extracts from the bark of Mapok at a concentration of 3000 ppm showed the highest inhibited percentage of germinating inhibition root length inhibition stem length inhibition and dry weight inhibition. 2. Fraction F8 of leaves ethyl acetate extract showed the highest inhibited percentage of germinating inhibition root length inhibition stem length inhibition and dry weight inhibition.

The objective of this research was to isolate plant growth promoting rhizobacteria from Wedelia trilobata (L.) A.S. Hitchcock rhizosphere and preliminarily test their plant growth promoting properties such as nitrogen fixation siderophore production phosphate solubilization and indole-3-acetic acid production. Forty-seven bacterial isolates were obtained including 26 isolates of gram positive 18 isolates of gram negative and 3 isolates of actinobacteria. After that 8 isolates (including BWTM1_MSU BWTM2_MSU BWTM5_MSU BWTM6_MSU BWWM6_MSU BWWM8_MSU BWWM9_MSU and BWWM16_MSU) were selected for studying on root colonization and plant growth promoting properties under aseptic condition. The results showed that BWTM5_MSU had the evident on the promotion of plant growth with the most percentage of rice seed germination (63.33%) high level of root and shoot length with 6.11 cm and 3.89 cm respectively. As a result the root and shoot fresh weight was as high as 9.56 mg and 11.33 mg respectively. It also correlated with the highest production of indole-3-acetic acid up to 421.29 ug/ml. The root colonization was studied by counting rhizobacteria isolate BWTM5_MSU from rice rhizoplane and rhizosphere with presented bacterial number at 9.67x106 CFU/g root and 1.64x10 10 CFU/g soil respectively.

In this study The paper aims to produce extracts from guava leaves. For use in food packaging. And to test their ability to inhibit bacteria. Staphylococcus aureus paper extracts from guava leaves. Because the bacteria Staphylococcus aureus to live in the air dust waste water food and milk supplies. The strain also grows well in climates with oxygen. The test extracts from guava leaves. A chemical properties of guava leaf extract has a pH of 5.4 + 0.02 acid content (% citric acid) was 1.2 ± 0.1 and the amount of phenolic total (mg gallic acid / g) levels. 63.1: 1.9 The performance of guava leaf extract in inhibiting bacteria. Staphylococcus aureus by means paper disc diffusion on agar Nutrient agar at 36 ° C for 24 hours diameter clear zone (cm.) Was 1.3 ± 0.5 and tested for the Minimum Inhibitory Concentration (MIC) by Broth Dilution Technique with. equal to 1.56% of extract of guava leaves

The purposes of this research were to assess the combined phytotoxicity of glyphosate and carbendazim which were normally used as herbicide and fungicide contaminated in soil as 100 – 1000 mg/kg on seedling growth of ridge gourd. Both of glyphosate and carbendazim were toxic to shoot and root growth of ridge gourd seedlings. The 50% effective concentration (EC50) on shoot length root length root fresh weight and shoot dried weight of ridge gourd seedlings for glyphosate were 669.4 413.0 574.5 and 241.4 mg/kg respectively whereas for carbendazim were >1000 357.8 >1000 and 71.4 mg/kg respectively. Co-contamination between glyphosate and carbendazim seemed to be antagonistic effect. Both of glyphosate and carbendazim at all concentration were not toxic to chlorophyll content in cotyledon of ridge gourd seedlings.